User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/04: Difference between revisions
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== | ==PCR with 2550/2178 primer pair at 60°C== | ||
Aim: see if these primers are better at sex differentiation than the p2/p8 ones | |||
method: | |||
same master mix as yesterday - one extra sample IZ - ethel | |||
All others corresond to the same letters as yesterday | |||
NB the 94°C soak went for 8.30 instead of 2 mins. | |||
Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C. | |||
Revision as of 21:54, 3 December 2012
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PCR with 2550/2178 primer pair at 60°CAim: see if these primers are better at sex differentiation than the p2/p8 ones method: same master mix as yesterday - one extra sample IZ - ethel All others corresond to the same letters as yesterday NB the 94°C soak went for 8.30 instead of 2 mins. Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.
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