User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09
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| - | == | + | ==Bird PCR== |
| - | + | Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. | |
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| + | Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. | ||
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| + | Master Mix: | ||
| + | 7 x lorikeets | ||
| + | 2 x positive controls (male female) | ||
| + | 1 negative control | ||
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| + | 20ul total volume | ||
Revision as of 19:06, 8 January 2013
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Bird PCRAim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control 20ul total volume
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