User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

Bird PCR

Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.

Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

  • 1ul F primer
  • 1ul R primer
  • 2.0ul dNTPs
  • 0.5u Taq
  • 1ul DNA
  • 2.5ul 10x buffer
  • 1.5ul MgCl2
  • 16ul H2O
  • 1:1SWWBD
  • 2:2SWWBD
  • 3:R5#9
  • 4:R5#51
  • 5:R5#52
  • 6:R5#100
  • 7 :Pink Gold

male female neg

Personal tools