User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15: Difference between revisions
From OpenWetWare
(Autocreate 2013/01/15 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Results== | ||
* | * last samples produced valid results when compared with positive controls | ||
Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using. | |||
Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples. | |||
Master Mix: | |||
7 x lorikeets | |||
2 x positive controls (male female) | |||
1 negative control | |||
*1ul F primer | |||
*1ul R primer | |||
*2.0ul dNTPs | |||
*0.5u Taq | |||
*1ul DNA | |||
*2.5ul 10x buffer | |||
*1.5ul MgCl<sub>2</sub> | |||
*16ul H<sub>2</sub>O | |||
*1:1SWWBD | |||
*2:2SWWBD | |||
*3:R5#9 | |||
*4:R5#51 | |||
*5:R5#52 | |||
*6:R5#100 | |||
*7 :Pink Gold | |||
male | |||
female | |||
neg | |||
Results: no bands. Try lower annealing temperature - say 55°C | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 01:16, 18 January 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Results
Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using. Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control
male female neg Results: no bands. Try lower annealing temperature - say 55°C | |
