User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15
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| - | == | + | ==Results== |
| - | * | + | * last samples produced valid results when compared with positive controls |
| + | |||
| + | Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using. | ||
| + | |||
| + | Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples. | ||
| + | |||
| + | Master Mix: | ||
| + | 7 x lorikeets | ||
| + | 2 x positive controls (male female) | ||
| + | 1 negative control | ||
| + | |||
| + | *1ul F primer | ||
| + | *1ul R primer | ||
| + | *2.0ul dNTPs | ||
| + | *0.5u Taq | ||
| + | *1ul DNA | ||
| + | *2.5ul 10x buffer | ||
| + | *1.5ul MgCl<sub>2</sub> | ||
| + | *16ul H<sub>2</sub>O | ||
| + | |||
| + | *1:1SWWBD | ||
| + | *2:2SWWBD | ||
| + | *3:R5#9 | ||
| + | *4:R5#51 | ||
| + | *5:R5#52 | ||
| + | *6:R5#100 | ||
| + | *7 :Pink Gold | ||
| + | male | ||
| + | female | ||
| + | neg | ||
| + | |||
Revision as of 03:58, 15 January 2013
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Results
Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using. Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control
male female neg
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