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'''Procedure 3: Bacteria Cell Morphology Observations''' | '''Procedure 3, part 1: Bacteria Cell Morphology Observations''' | ||
To observe the bacteria from our sample plates, we used a toothpick to pick up a colony of bacteria from 4 plates (10^-3 with tet, 10^-3 without, 10^-5 with tet, 10^-5 without tet) and put them on slides with a drop of water. We placed a cover slip on top and used a compound microscope to take a closer look at the shape and mobility of the bacteria. Below are the images we collected: | To observe the bacteria from our sample plates, we used a toothpick to pick up a colony of bacteria from 4 plates (10^-3 with tet, 10^-3 without, 10^-5 with tet, 10^-5 without tet) and put them on slides with a drop of water. We placed a cover slip on top and used a compound microscope to take a closer look at the shape and mobility of the bacteria. Below are the images we collected, all at 40x magnification: | ||
[[Image:IMG_1793.jpg|thumb|center|plate 10^-3 without, shape: coccus (sphere), motility: individual cells moving individually diagonally from top right to bottom left]] | [[Image:IMG_1793.jpg|thumb|center|plate 10^-3 without, shape: coccus (sphere), motility: individual cells moving individually diagonally from top right to bottom left]] | ||
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[[Image:IMG_1808.jpg|thumb|center|place 10^-5 with tet, no bacteria present]] | [[Image:IMG_1808.jpg|thumb|center|place 10^-5 with tet, no bacteria present]] | ||
'''Procedure 3, part 2: Gram Stain''' | |||
To perform the gram stain, we had to first make a wet mount by sterilizing an inoculation loop | |||
Revision as of 12:26, 4 February 2016
Caroline Sell
Bio 210 - 007
Lab 4: 02-04-16
Lab 3: 01-28-16
Before starting today's procedures, we took one last look at the Hay Infusion Culture. As of today, it has been sitting for 2 weeks. There were no changes in smell but the appearance did change. All the organisms we saw growing at each layer were nearly gone. There was a lot of dirt that had sunk to the bottom, and the top layer was extremely reduced. We observed that 50% of the water had evaporated. We hypothesize that there was a change in appearance because there were no more nutrients left in the culture and everything died. However, we did see that there was growth on our agar plates because there were nutrients present allowing bacteria to cultivate.
Procedure 1: Quantifying and Observing Microorganisms
We used colony morphology to help determine the type of bacteria growing on our plates. We counted the colonies on each plate, and made sure we divided by 100 to keep the units the same (colonies/um) since we made the cultures with 100 microliters.
According to this table, there were no results for the 10^-5 tet, 10^-7 with and without tet, and 10^9 with and without tet plates.
Procedure 2: Antibiotic Resistance
Based on the table above and the images of the plates below, we saw that for the previously mentioned plates, there was no growth at all. The only plates we could compare tet/no tet were the 10^-3 plates and we saw that the growth looked slightly different. The colonies on the plate with tet were larger, more spread out, and brighter in color whereas the colonies on the plate without tet were smaller and covered more of the plate. Neither of these was quite enough to be a lawn. The differences in colony type could indicate that the antibiotic did not allow for as many smaller colonies to grow as we saw with the plate without tet. As seen in the table above, there were significantly fewer colonies on the plate with tet than on the plate without tet. There appeared to be presence of some fungi on each of the plates where there was growth.
Tetracycline is a broad-spectrum antibiotic that inhibits the growth of
Procedure 3, part 1: Bacteria Cell Morphology Observations
To observe the bacteria from our sample plates, we used a toothpick to pick up a colony of bacteria from 4 plates (10^-3 with tet, 10^-3 without, 10^-5 with tet, 10^-5 without tet) and put them on slides with a drop of water. We placed a cover slip on top and used a compound microscope to take a closer look at the shape and mobility of the bacteria. Below are the images we collected, all at 40x magnification:
Procedure 3, part 2: Gram Stain
To perform the gram stain, we had to first make a wet mount by sterilizing an inoculation loop
Lab 2: 01-21-16
With the Hay Infusion Culture we made last week in lab, we made wet mounts for microscopic observation of the culture. The Hay Infusion Culture consisted of 10-12 grams of soil/ground vegetation from the sample we took last week, 0.1 grams of dried milk, and then everything was mixed together. The top of the jar was left open for one week.
First, we observed the Hay Infusion Culture in the jar. There was no particular smell. We did notice a bit of mold on the top of the culture.
We noticed some plant matter (algae) near the top of the culture and this would be because of the need to perform photosynthesis. Along with the algae at the top level we also saw Paramecium, which would need the nutrients to survive.
Below are images of the organisms found at each level (top, middle, and bottom) along with their size and name.
. . .
Paramecium: As per the Freeman text, paramecium are protists that are motile. They are a single cell, part of the Alveota lineage, and have 1 macro and 1 micro nuclei. They can reproduce asexually by binary fission or sexually with conjugation. In order for them to survive, they must consume the nutrients surrounding them in their environment, which is why we found them with the diatoms at the top of our culture.
If the Hay Infusion "grew" for another two months, we would expect more growth of algae such as diatoms. We already saw these on each level of our culture. However, there was only 0.1 grams of dried milk and at some point the nutrients would run out and nothing else would be able to grow in the culture.
. . .
We also prepared and plated serial dilutions using our Hay Infusion Culture.
CLS
Lab 1: 01-14-16
We observed Transect #2, located by the Amphitheater and Hughes/McDowell Hall. There is a creek, many rocks, bushes, and trees. Below are images and a diagram of the transect. For this week's activity, we collected samples of all elements found in the transect to make a Hay Infusion Culture in lab.
Biotic: bush, trees, moss in water
Abiotic: rocks, sticks, dead leaves
CLS
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