User:Sung-Hye Grieco

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[[Image:IMG_0858.jpg|thumb|right|Photo-synthetic bacteria set up in fermenter suite]]
 
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=='''Sung-Hye H. Grieco'''==
 
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* Suite Manager, [http://www.cbr.ubc.ca/fermenter.htm Single Cell Fermenter Suite], [http://www.cbr.ubc.ca Centre for Blood Research], [http://www.ubc.ca University of British Columbia]
 
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*Contact info via [[Special:Emailuser/Sung-Hye Grieco|E-mail]]
 
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*Lab website:[http://www.cbr.ubc.ca/fermenter.htm http://cbr.ubc.ca/fermenter.htm]
 
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I work in the [http://www.cbr.ubc.ca Centre for Blood Research] at University of British Columbia.  I learned about [[OpenWetWare]] after I attended [http://www.bio.org/ind/pacrim/08/ Pacific Rim Summit on Industrial Biotechnology and Bioenergy] meeting at Vancouver in 2008 September and listened the inspiring plenary lecture by [[User:Endy|Dr. Endy Drew]] about [[Synthetic_Biology|Synthetic Biology]] titled <em> "Can we make Biology Easy to Engineer?"</em>, and I've joined because I wanted to share protocols and ideas about sciences and discuss about daily questions for myself and others.. After I joined it, I also realized that I can use this OpenWetWare for live website for communicating with fermenter users for on-going project using OpenWetWare [[Lab_notebook|LabNotebook]].
 
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=='''Research interests'''==
 
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# <font color=red> Small-scale fermentation optimization and process development</font>: <br> Provide time and cost efficient production tools using fermentation equipment.
 
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# <font color=red> Building up fermentation data base</font>:<br> Provide on-line database from fermentation conducted from our suite with detailed fermentation conditions for result download, invoices, tracking down previous data, and data comparison and easy analysis.
 
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# <font color=red> Sharing scientific information for trouble shooting and protocal exchanges</font>:<br> Also provide information about basic materials (media composition, characteristics of host strains), instructions on how to prepare bioreactors, conducting fermentation, and analysis of fermentation data and off-line data.
 
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=='''Work Experiences'''==
 
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* 2007-Present, Suite manager, <em> University of British Columbia </em>, Centre for Blood Research, Fermentation Suite, Vancouver, BC, Canada
 
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* 2005-2006, Research Associate, <em> University of British Columbia </em>, Department of Biochemistry and Molecular Biology, Vancouver, BC, Canada
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Design of 1-step and 2-step FRET sensors for PA Fur for study of structural change in metal binding and ligand (metal ions and DNA) binding (2006).
 
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=='''Education'''==
 
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* 2004-2005, Post doctoral resesarch, <em> University of British Columbia </em>, Department of Biochemistry and Molecular Biology, Vancouver, BC, Canada
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Design of monomeric form of Pseudomonas Aeruginosa ferric uptake regulator (Fur) using mutagenesis and chemical modification of cystein residue (2005). 
 
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* 2001-2003, Post doctoral resesarch, <em> Harvard Medical School </em>, Center for Biochemical and Biophysical Sciences and Medicine (Currently Department of Pathology), Boston, MA, USA
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Optical imaging of hMT-2 FRET sensors in a human liver cells and establishment of its uptake and recycle mechanism in vivo. (2006)<br>
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Design of FRET sensors for hMT-2 (human metallothionein-2) (2003). <br>
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Design and purification of each domain of human metallothionein-2 in E.coli system (2003)<br>
 
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* 1997-2001, PhD. Bioengineering, <em> Osaka University </em>, Department of  Biotechnology, Osaka, Japan
 
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&nbsp;&nbsp;&nbsp;&nbsp;<i>Doctoral dissertation: Production and Characterization of Human Metallothionein and its Domains</i><br>
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Solving on-going problem of purification and characterization of hMT-2 and establishment of single step purification with the highest production system up to date for hMT-2 (2001).<br>
 
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&nbsp;&nbsp;&nbsp;&nbsp;• Construction of oligomeric forms of human metallothionein-2 in E.coli and development of purification and characterization protocols with functional activity (metal binding capacity) (1999).
 
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* 1995-1997, MS. Biochemistry, <em> Catholic Medical School </em>, Department of Biochemistry, Seoul, Korea
 
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=='''Publications'''==
 
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<biblio>
 
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#Paper6 pmid=19221805
 
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#Paper5 pmid=17111383
 
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#Paper4 pmid=15911539
 
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#Paper3 pmid=12618543
 
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#Paper2 pmid=11162412
 
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#Paper1 pmid=10952009
 
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</biblio>
 
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=='''Useful links'''==
 
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*[[User:Fermenter_User|Online Lab Notebook for Fermenter Users]]
 
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*[[Help|Notebook Lab Notebook]]<br><br>
 
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*[[OpenWetWare:Welcome|Introductory tutorial]]
 
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*[[Help|OpenWetWare help pages]]
 
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#[http://meta.wikimedia.org/wiki/MediaWiki_User%27s_Guide Editing User Guide (meta.wikimedia.org)]
 
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#[[Help:Wiki_questions|Ask a Question about Editing-Related Questions]]
 
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#[http://meta.wikimedia.org/wiki/Help:Advanced_editing Advanced Editing (meta.wikimedia.org)]
 
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#[http://meta.wikimedia.org/wiki/Help:Table How to make wiki table and insert pictures (meta.wikimedia.org)]
 
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#[http://www.mediawiki.org/wiki/Manual:Administrators#Protection How to protect your page from editing by unauthorized users(mediawiki.org)]
 
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#[http://www.mediawiki.org/wiki/Help:Magic_words Magic Words(mediawiki.org)]
 
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#[http://meta.wikimedia.org/wiki/Help:Images_and_other_uploaded_files Table How to make wiki table and insert pictures (meta.wikimedia.org)]
 
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=='''Memo'''==
 
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#How to delete page: after {{ sign, type [deleteme] and finish with }} sign.
 

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