User:Sung-Hye Grieco

Photo-synthetic bacteria set up in fermenter suite

Sung-Hye H. Grieco

• Suite Manager, Single Cell Fermenter Suite, Centre for Blood Research, University of British Columbia
• Contact info via E-mail
• Lab website:http://cbr.ubc.ca/fermenter.htm

I work in the Centre for Blood Research at University of British Columbia. I learned about OpenWetWare after I attended Pacific Rim Summit on Industrial Biotechnology and Bioenergy meeting at Vancouver in 2008 September and listened the inspiring plenary lecture by Dr. Endy Drew about Synthetic Biology titled "Can we make Biology Easy to Engineer?", and I've joined because I wanted to share protocols and ideas about sciences and discuss about daily questions for myself and others.. After I joined it, I also realized that I can use this OpenWetWare for live website for communicating with fermenter users for on-going project using OpenWetWare LabNotebook.

Research interests

1. Small-scale fermentation optimization and process development:
   Provide time and cost efficient production tools using fermentation equipment.
2. Building up fermentation data base:
   Provide on-line database from fermentation conducted from our suite with detailed fermentation conditions for result download, invoices, tracking down previous data, and data comparison and easy analysis.
3. Sharing scientific information for trouble shooting and protocal exchanges:
   Also provide information about basic materials (media composition, characteristics of host strains), instructions on how to prepare bioreactors, conducting fermentation, and analysis of fermentation data and off-line data.

Work Experiences

• 2007-Present, Suite manager, University of British Columbia , Centre for Blood Research, Fermentation Suite, Vancouver, BC, Canada

• 2005-2006, Research Associate, University of British Columbia , Department of Biochemistry and Molecular Biology, Vancouver, BC, Canada

    • Design of 1-step and 2-step FRET sensors for PA Fur for study of structural change in metal binding and ligand (metal ions and DNA) binding (2006).

Education

• 2004-2005, Post doctoral resesarch, University of British Columbia , Department of Biochemistry and Molecular Biology, Vancouver, BC, Canada

    • Design of monomeric form of Pseudomonas Aeruginosa ferric uptake regulator (Fur) using mutagenesis and chemical modification of cystein residue (2005).

• 2001-2003, Post doctoral resesarch, Harvard Medical School , Center for Biochemical and Biophysical Sciences and Medicine (Currently Department of Pathology), Boston, MA, USA

    • Optical imaging of hMT-2 FRET sensors in a human liver cells and establishment of its uptake and recycle mechanism in vivo. (2006)
    • Design of FRET sensors for hMT-2 (human metallothionein-2) (2003).
    • Design and purification of each domain of human metallothionein-2 in E.coli system (2003)

• 1997-2001, PhD. Bioengineering, Osaka University , Department of Biotechnology, Osaka, Japan

    Doctoral dissertation: Production and Characterization of Human Metallothionein and its Domains
    • Solving on-going problem of purification and characterization of hMT-2 and establishment of single step purification with the highest production system up to date for hMT-2 (2001).
    • Construction of oligomeric forms of human metallothionein-2 in E.coli and development of purification and characterization protocols with functional activity (metal binding capacity) (1999).

• 1995-1997, MS. Biochemistry, Catholic Medical School , Department of Biochemistry, Seoul, Korea

Publications

1. Grieco SH, Lee S, Dunbar WS, MacGillivray RT, and Curtis SB. Maximizing filamentous phage yield during computer-controlled fermentation. Bioprocess Biosyst Eng. 2009 Oct;32(6):773-9. DOI:10.1007/s00449-009-0303-3 |
2. Hao Q, Hong SH, and Maret W. Lipid raft-dependent endocytosis of metallothionein in HepG2 cells. J Cell Physiol. 2007 Feb;210(2):428-35. DOI:10.1002/jcp.20874 |
3. Hong SH, Hao Q, and Maret W. Domain-specific fluorescence resonance energy transfer (FRET) sensors of metallothionein/thionein. Protein Eng Des Sel. 2005 Jun;18(6):255-63. DOI:10.1093/protein/gzi031 |
4. Hong SH and Maret W. A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2255-60. DOI:10.1073/pnas.0438005100 |
5. Hong S, Toyama M, Maret W, and Murooka Y. High yield expression and single step purification of human thionein/metallothionein. Protein Expr Purif. 2001 Feb;21(1):243-50. DOI:10.1006/prep.2000.1372 |
6. Hong SH, Gohya M, Ono H, Murakami H, Yamashita M, Hirayama N, and Murooka Y. Molecular design of novel metal-binding oligomeric human metallothioneins. Appl Microbiol Biotechnol. 2000 Jul;54(1):84-9. DOI:10.1007/s002530000360 |

7. Hong SH, Jeong SY, Ahn SG, Hwang IS and Kim IK. Active adenosine transporter-mediated adenosine entrance into HL-60 cells leads to the induction of apoptosis through down-regulation of c-Myc. Experimental and Molecular Medicine, 1997 Sep; 29(3) 183-190.

   File:Emm29-3-8.pdf
   

Useful links

• Online Lab Notebook for Fermenter Users
• Notebook Lab Notebook
  
  

• Introductory tutorial
• OpenWetWare help pages

1. Editing User Guide (meta.wikimedia.org)
2. Ask a Question about Editing-Related Questions
3. Advanced Editing (meta.wikimedia.org)
4. How to make wiki table and insert pictures (meta.wikimedia.org)
5. How to protect your page from editing by unauthorized users(mediawiki.org)
6. Magic Words(mediawiki.org)
7. Table How to make wiki table and insert pictures (meta.wikimedia.org)

Memo

1. How to delete page: after {{ sign, type [deleteme] and finish with }} sign.