User:Sunny Sharma

Contact Info

• Sunny Sharma, PhD
• Massachusetts General Hospital – Cancer Centre, Boston, MA.
• Email me through OpenWetWare

Research Fellow at MGH – Cancer Centre, Boston, MA.

Education

• Research Associate at Institute of Molecular Biosciences, Goethe University, Frankfurt am Main, Germany [1]
• 2014, PhD (Biology), Goethe University, Frankfurt am Main, Germany [2]
• 2007, MS (Molecular Biology & Biochemistry), Guru Nanak Dev University, Amritsar, India [3]
• 2005, BS, Bangalore University, Bangalore, India [4]

Awards

EMBO (European Molecular Biology Organisation) Long Term Fellowship. (2014-2016)[5]

DAAD (Deutsche Akademische Austauschdienst) PhD scholarship (2010 - 2013) [6]

CEF (Excellence Cluster-Frankfurt am Main) PhD fellowship (2009 - 2010) [7]

ICMR (Indian Council of Medical Research) Junior Research Fellowship (2009 - 2010) [8]

Research interests

1. Ribosome Biogenesis
2. RNA modifications
3. Developmental Biology
4. Immunology
5. Gerontology

Publications

16) Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation.[9]

15) Mapping of Complete Set of Ribose and Base Modifications of Yeast rRNA by RP-HPLC and Mung Bean Nuclease Assay.[10]

14) Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function.[11]

13) Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans. [12]

12)‘View From A Bridge’: A New Perspective on Eukaryotic rRNA Base Modification.[13]

11) The reverse transcription signature of N-1-methyladenosine in RNA-Seq is sequence dependent.[14]

10) Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1.[15]

9) Identification of a new ribose methylation in the 18S rRNA of S. cerevisiae.[16]

8) Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.[17]

7) Absolute and relative quantification of RNA modifications via biosynthetic isotopomers.[18]

6) Partial methylation at Am100 in 18S rRNA of baker´s yeast shows ribosome heterogeneity on the level of eukaryotic rRNA modification. Plos One [19]

5) Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae.[20]

4) Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively.[21]

3) Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in Saccharomyces cerevisiae.[22]

2) Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA.[23]

1) Association of P2X7 receptor +1513 (A-->C) polymorphism with tuberculosis in a Punjabi population.[24]

Conferences/ Workshops

1. Plenary talk at 10th Ribosome Synthesis meeting 19th-25th August, Brussels.[25]
2. Seminar at RNA club, 16th October 2013 [26]
3. Plenary talk at Yeast Meeting, Frankfurt 2013 [27]
4. Poster presentation at 18th Annual RNA meeting of the RNA society, Davos, Switzerland [28]
5. Seminar at RNA club, 26th September 2012 [29]
6. Poster presentation at 9th International Conference on Ribosome Synthesis, Banff, Canada [30]

Useful links

• http://www.muk.uni-frankfurt.de/53895688/033?
• http://www.yeastgenome.org/
• http://www.rnaclub.de
• http://people.biochem.umass.edu/fournierlab/3dmodmap/main.php
• http://modomics.genesilico.pl/
• http://www.rna.icmb.utexas.edu/
• http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
• http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index