User:Sydney Marshall/Notebook/Protease Project/2016/03/22

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Objective

  • Set up silver and gold nanoparticle synthesis reactions with protein varieties
  • Begin Bacterial Disk Diffusion Test on nanoparticles that were successfully synthesized

Procedure and Observations

Metalloprotein Combination Concentration (x) Disc Diameter (mm) Notes
Soy-Silver (SS)
1 1 6
2 0.5 4
3 0.25 0
4 0.125 0
5 0.0625 0
Pea-Silver (PS) Concentration (x)
1 1 0
2 0.5 0
3 0.25 0
4 0.125 0
5 0.0625 0
Casein-Silver (CS) Concentration (x)
1 1 10
2 0.5 NA bacteria not spread evenly
3 0.25 13
4 0.125 0
5 0.0625 0
Soy-Gold (SG) Concentration (x)
1 1 12
2 0.5 (6smallest, 11largest) non discernable diameter
3 0.25 16
4 0.125 10
5 0.0625 13 disc not in center of circle
Whey-Gold (WG) Concentration (x) non discernable diameter
1 1 11
2 0.5 7
3 0.25 15
4 0.125 0
5 0.0625 0
Rice-Silver (RS) Concentration (x)
1 1 10
2 0.5 0
3 0.25 0
4 0.125 0
5 0.0625 0
Ampicillin (A) Concentration (ug/uL)
1 0.064 28
2 0.032 18
3 0.016 14
4 0.008 7
5 0.004 0
Whey Silver Concentration (ug/uL)
1 0.064 20 indiscernable blob
2 0.032 19
3 0.016 0
4 0.008 0
5 0.004 0

Results