User:Sydney Murphy/Notebook/Biology 210 at AU: Difference between revisions
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We began by making a wet mount of a mix of known organisms and classifying two using a dichotomous key. Our slide contained Paramecium Multimicronucleature at 250 micrometers and Colpidium at 50 micrometers. We then moved onto out Hay Infusion Culture, which had separated, was beginning to grow mold on the surface, and was giving off a pungent odor. We took a sample from the center bottom, the center top, and the center middle next to a leaf and made wet mounts of the three, identifying two organisms from each area. When this was finished we set up next weeks experiment by making serial dilutions, beginning with 100 microliters of Hay Culture: 10 milliliters Broth then diluting 100 microliters of this solution into 10 milliliters of Broth. This continued two more times, leaving us with 4 tubes of decreasing concentration. We then spread 100 microliters of each solution on two agar plates, one with tetracycline and one without. The plates were then set to grow agar side up for a week. | We began by making a wet mount of a mix of known organisms and classifying two using a dichotomous key. Our slide contained Paramecium Multimicronucleature at 250 micrometers and Colpidium at 50 micrometers. We then moved onto out Hay Infusion Culture, which had separated, was beginning to grow mold on the surface, and was giving off a pungent odor. We took a sample from the center bottom, the center top, and the center middle next to a leaf and made wet mounts of the three, identifying two organisms from each area. When this was finished we set up next weeks experiment by making serial dilutions, beginning with 100 microliters of Hay Culture: 10 milliliters Broth then diluting 100 microliters of this solution into 10 milliliters of Broth. This continued two more times, leaving us with 4 tubes of decreasing concentration. We then spread 100 microliters of each solution on two agar plates, one with tetracycline and one without. The plates were then set to grow agar side up for a week. | ||
[[Image:Diagram_1.jpg]] | |||
Within the Hay Culture Infusion there was little variation between the layers. Each layer consisted of Spirostomum varying in length from 550 Micrometers to 1100 micrometers. The two lower levels both consisted of Stenor, ranging from 47.5 micrometers to 52.5 micrometers. The top also consisted of a Colpidium at 52.5 micrometers. The lack of diversity within the Culture is likely due to the cold weather and the fact that the soil they were collected from was frozen when the samples were taken. There will likely be more diversity when the weather heats up and the ground thaws out. | Within the Hay Culture Infusion there was little variation between the layers. Each layer consisted of Spirostomum varying in length from 550 Micrometers to 1100 micrometers. The two lower levels both consisted of Stenor, ranging from 47.5 micrometers to 52.5 micrometers. The top also consisted of a Colpidium at 52.5 micrometers. The lack of diversity within the Culture is likely due to the cold weather and the fact that the soil they were collected from was frozen when the samples were taken. There will likely be more diversity when the weather heats up and the ground thaws out. |
Revision as of 00:50, 28 January 2015
January 27, 2015
Protist Identification and Using a Dichotomous Key: January 21, 2015
This lab introduced us to using Dichotomous keys and allowed us to find and classify the protists in our Hay Culture Infusions. It is likely that the Culture will contain a large number of protists throughout the span of the jar, due to the different types of soils collected.
We began by making a wet mount of a mix of known organisms and classifying two using a dichotomous key. Our slide contained Paramecium Multimicronucleature at 250 micrometers and Colpidium at 50 micrometers. We then moved onto out Hay Infusion Culture, which had separated, was beginning to grow mold on the surface, and was giving off a pungent odor. We took a sample from the center bottom, the center top, and the center middle next to a leaf and made wet mounts of the three, identifying two organisms from each area. When this was finished we set up next weeks experiment by making serial dilutions, beginning with 100 microliters of Hay Culture: 10 milliliters Broth then diluting 100 microliters of this solution into 10 milliliters of Broth. This continued two more times, leaving us with 4 tubes of decreasing concentration. We then spread 100 microliters of each solution on two agar plates, one with tetracycline and one without. The plates were then set to grow agar side up for a week.
Within the Hay Culture Infusion there was little variation between the layers. Each layer consisted of Spirostomum varying in length from 550 Micrometers to 1100 micrometers. The two lower levels both consisted of Stenor, ranging from 47.5 micrometers to 52.5 micrometers. The top also consisted of a Colpidium at 52.5 micrometers. The lack of diversity within the Culture is likely due to the cold weather and the fact that the soil they were collected from was frozen when the samples were taken. There will likely be more diversity when the weather heats up and the ground thaws out.
Sydney Murphy
January 27, 2015
Volvocine Line and Transect Introduction: January 14, 2015
The purpose of this lab was to reintroduce us to microscopes and making wet mounts, as well as getting acquainted with our transects. By taking the first day to adjust to being back and introducing us to our transects it allows us to successfully transition into the class and get to know our partners better.
We began the lab by making wet mount slides of the three examples of the Volvocine line: Chlamydomonas, Gonium, and Volvox. We then observed the samples under a microscope, searching specifically for colony size, cell specialization and motility mechanisms. After, we walked around campus and were introduced to our transects, which we mapped, drew samples of and listed biotic and abiotic factors. When we returned we made a Hay Infusion Culture by combining 12 grams of our soil, 500 mLs of water, .1 grams of dried milk in a jar, they were mixed together and left to sit open for a week on the back table.
Characteristic | Chlamydomonas | Gonium | Volvox |
---|---|---|---|
Number of cells | 1 | 4 | 1 |
Colony Size | 10 | 17.5 | 200 |
Specialization? | No | Yes | Yes |
Motility Mechanisms | Flagella | None | Flagella |
Iso or Oogamous? | Isogamous | Oogamous | Oogamous |
The side by side comparison of the Volvocine line shows the evolution over time, from a single-celled organism, to a colony, to an advance organism.
Abiotic | Biotic |
Snow | Brussel Sprouts |
Rocks | Clover |
Irrigation Tubes | Pine Needles |
Plastic label | Bushes |
Paint | Grass |
These are some of the prominent Abiotic and Biotic factors from our Transect. Transect four is found within the community garden near the soccer fields towards the back edge of campus. It is a fairly flat area with four raised plant beds on the one side and three bushes on the other side, closer to the fence and gate.
Overall, this experiment worked well; we were able to observe our transect and collect a variety of soil for our Hay Infusion Culture. Though, the ground was frozen and the protists we collected may end up reflecting this in their diversity, or lack thereof. Hopefully it will be warmer next time we collect from our transects, allowing us more variety in protists collected.
Sydney Murphy
January 22,2015
This is my test message for lab! Hope it works well!