User:TChan: Difference between revisions

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==Wed 6.14.06==
<div class="tabs-blue">
<ul>
<li id="current">[[TChan|Project Overview]]</li>
<li>[[TChan/Schematics|Schematics]]</li>
<li>[[TChan/OligoList|Oligo List]]</li>
<li>[[TChan/Scaffold|Scaffold]]</li>
<li>[[TChan/Images|Images]]</li>
<li>[[TChan/iGEMNotes|iGEM Notes]]</li>
</ul>
</div>
<br style="clear:both">
<br>


==Tu 6.13.06==
*See [[IGEM:Harvard/2006/DNA_nanostructures|iGEM 2006 Page]] for more info
1. Picked Colonies of Existing BioBrick Transformants
*Picked:
  3 colonies of E7,
  2 colonies of E0,
  2 colonies of R0, and numbered accordingly


2. Ran Gel of DNA Nanostructures
*Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V.  (NB: Only left four lanes are ours.)
  [[Image:Tc_mm_pt_lh_folding_0613.jpg|thumb|left|Nanostructure Gel]]
 
  1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold
 


==iGEM 2006 Notebook==
<calendar>
name=IGEM:Harvard/2006/DNA_nanostructures/Notebook
date=2006/07/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>


<calendar>
name=IGEM:Harvard/2006/DNA_nanostructures/Notebook
date=2006/10/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>






==Notebook==
<calendar>
name=TChan/Notebook
date=2006/2/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>


<calendar>
name=TChan/Notebook
date=2006/5/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>








==M 6.12.06==
==Contact Info==
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA
   Tiffany Chan
*Three reactions were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures  given protocol].
   Biochemical Sciences
oligos + scaffold
   Kirkland '07
-oligos
   Academic Interests: DNA nanostructures, non-coding RNAs (SO. HOT.), drug design and delivery
-scaffold
   [mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]
 
  Currently taking [http://openwetware.org/wiki/Harvard:Biophysics_101/2007 Biophysics 101]
2. Transformed Bacteria with Existing BioBrick Plasmids
*Transformed into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
   R0010 (lac operon promoter),
   E7104 (T4 promoter + gfp),
   and E0241 (gfp).
 
===Top10 Transformation===
   Thaw cells; aliquot out ~30uL per transformation.
  Add desired amount of plasmid DNA (1 uL in this case?) to cells and leave on ice for 30 min.
  Heat shock at 37&degC for 30 sec in a heating block. 
  Ice for 2 min.
  Shake in 37%deg incubator for 1 hr.
  Pour onto plates with the required antibiotic (carbinomycin in this case); spread with beads or plate spreader.
   Incubate plates in 37%deg incubator overnight.
 
 
HOMEWORK:
1) Past iGEM Projects Presentation: UCSF
2) Prospective Projects Research:
a) Lactobacillus Hijacking
b) Receptor-based DNA Nanostructure Latch
c) Biocryptography w/ DNA Nanostructures
d) ?
 
==Email==
[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]

Latest revision as of 13:12, 9 April 2009




iGEM 2006 Notebook

<calendar> name=IGEM:Harvard/2006/DNA_nanostructures/Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=IGEM:Harvard/2006/DNA_nanostructures/Notebook date=2006/10/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>


Notebook

<calendar> name=TChan/Notebook date=2006/2/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=TChan/Notebook date=2006/5/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>



Contact Info

 Tiffany Chan
 Biochemical Sciences
 Kirkland '07
 Academic Interests: DNA nanostructures, non-coding RNAs (SO. HOT.), drug design and delivery
 chan.tiffany at gmail.com
 Currently taking Biophysics 101
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:TChan&oldid=300657"