User:TChan

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M 6.12.06
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==
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==M 6.12.06==
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1. Folded DNA Nanostructures
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<b>Goal:</b> Create nanotubes using Shawn's pre-designed oligo staples, and two controls: one without the scaffold, one without the staples
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*According to [http://openwetware.org/wiki/Folding_DNA_nanostructures  protocol], 3 combinations of components were mixed and thermocycled.
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2. Transformed Bacteria with Existing BioBrick Plasmids
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<b>Goal:</b> Transform existing BioBrick-containing plasmids in order to produce large enough quantities of DNA to work with later.
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*R0010 (lac operon promoter),
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*E7104 (T4 promoter + gfp),
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*and E0241 (gfp)
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were transformed into OneShot Top10 competent cells (Invitrogen) according to
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HOMEWORK:
HOMEWORK:
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d) ?
d) ?
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18:50, 12 June 2006 (EDT)
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==Email==
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Email: [mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]
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[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]

Revision as of 14:49, 14 June 2006

==

M 6.12.06

1. Folded DNA Nanostructures Goal: Create nanotubes using Shawn's pre-designed oligo staples, and two controls: one without the scaffold, one without the staples

  • According to protocol, 3 combinations of components were mixed and thermocycled.

2. Transformed Bacteria with Existing BioBrick Plasmids Goal: Transform existing BioBrick-containing plasmids in order to produce large enough quantities of DNA to work with later.

  • R0010 (lac operon promoter),
  • E7104 (T4 promoter + gfp),
  • and E0241 (gfp)

were transformed into OneShot Top10 competent cells (Invitrogen) according to



HOMEWORK: 1) Past iGEM Projects Presentation: UCSF 2) Prospective Projects Research: a) Lactobacillus Hijacking b) Receptor-based DNA Nanostructure Latch c) Biocryptography w/ DNA Nanostructures d) ?

Email

chan.tiffany at gmail.com

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