User:TChan
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(Difference between revisions)
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| - | == | + | ==Wed 6.14.06== |
==Tu 6.13.06== | ==Tu 6.13.06== | ||
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2. Ran Gel of DNA Nanostructures | 2. Ran Gel of DNA Nanostructures | ||
| - | *Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V. | + | *Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V. (NB: Only left four lanes are ours.) |
[[Image:Tc_mm_pt_lh_folding_0613.jpg|thumb|left|Nanostructure Gel]] | [[Image:Tc_mm_pt_lh_folding_0613.jpg|thumb|left|Nanostructure Gel]] | ||
| - | 1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold | + | 1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold |
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==M 6.12.06== | ==M 6.12.06== | ||
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1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA | 1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA | ||
*Three reactions were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures given protocol]. | *Three reactions were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures given protocol]. | ||
Revision as of 15:45, 14 June 2006
Contents |
Wed 6.14.06
Tu 6.13.06
1. Picked Colonies of Existing BioBrick Transformants
- Picked:
3 colonies of E7, 2 colonies of E0, 2 colonies of R0, and numbered accordingly
2. Ran Gel of DNA Nanostructures
- Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V. (NB: Only left four lanes are ours.)
1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold
M 6.12.06
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA
- Three reactions were mixed according to given protocol.
oligos + scaffold -oligos -scaffold
2. Transformed Bacteria with Existing BioBrick Plasmids
- Transformed into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
R0010 (lac operon promoter), E7104 (T4 promoter + gfp), and E0241 (gfp).
Top10 Transformation
Thaw cells; aliquot out ~30uL per transformation. Add desired amount of plasmid DNA (1 uL in this case?) to cells and leave on ice for 30 min. Heat shock at 37°C for 30 sec in a heating block. Ice for 2 min. Shake in 37%deg incubator for 1 hr. Pour onto plates with the required antibiotic (carbinomycin in this case); spread with beads or plate spreader. Incubate plates in 37%deg incubator overnight.
HOMEWORK:
1) Past iGEM Projects Presentation: UCSF
2) Prospective Projects Research:
a) Lactobacillus Hijacking
b) Receptor-based DNA Nanostructure Latch
c) Biocryptography w/ DNA Nanostructures
d) ?
