User:TChan

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==Wed 6.14.06==
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==W 6.14.06==
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1.
==Tu 6.13.06==
==Tu 6.13.06==
1. Picked Colonies of Existing BioBrick Transformants
1. Picked Colonies of Existing BioBrick Transformants
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   2 colonies of E0,  
   2 colonies of E0,  
   2 colonies of R0, and numbered accordingly
   2 colonies of R0, and numbered accordingly
 +
*Grew each colony in 5mL LB + 5uL of 50mg/mL Amp (1:100 dilution).
2. Ran Gel of DNA Nanostructures
2. Ran Gel of DNA Nanostructures
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   1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold
   1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold
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==M 6.12.06==
==M 6.12.06==
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA
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*Three reactions were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures  given protocol].
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*Three reactions below were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures  given protocol].
  oligos + scaffold
  oligos + scaffold
  -oligos
  -oligos
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2. Transformed Bacteria with Existing BioBrick Plasmids
2. Transformed Bacteria with Existing BioBrick Plasmids
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*Transformed into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
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*Transformed each BioBrick plasmid below into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
   R0010 (lac operon promoter),
   R0010 (lac operon promoter),
   E7104 (T4 promoter + gfp),  
   E7104 (T4 promoter + gfp),  
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HOMEWORK:
HOMEWORK:
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1) Past iGEM Projects Presentation: UCSF
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#Past iGEM Projects Presentation: UCSF
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2) Prospective Projects Research:
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#Prospective Projects Research:
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a) Lactobacillus Hijacking
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  Lactobacillus Hijacking
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b) Receptor-based DNA Nanostructure Latch  
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  Receptor-based DNA Nanostructure Latch  
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c) Biocryptography w/ DNA Nanostructures
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  Biocryptography w/ DNA Nanostructures
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d) ?
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  ?
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==Email==
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==Contact Info==
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[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]
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/Tiffany Chan
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/Cell: 781 330 1969
 +
/[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]

Revision as of 15:50, 14 June 2006

Contents

W 6.14.06

1.

Tu 6.13.06

1. Picked Colonies of Existing BioBrick Transformants

  • Picked:
 3 colonies of E7, 
 2 colonies of E0, 
 2 colonies of R0, and numbered accordingly
  • Grew each colony in 5mL LB + 5uL of 50mg/mL Amp (1:100 dilution).

2. Ran Gel of DNA Nanostructures

  • Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V. (NB: Only left four lanes are ours.)
Nanostructure Gel
Nanostructure Gel
 1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold






M 6.12.06

1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA

oligos + scaffold
-oligos
-scaffold

2. Transformed Bacteria with Existing BioBrick Plasmids

  • Transformed each BioBrick plasmid below into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
 R0010 (lac operon promoter),
 E7104 (T4 promoter + gfp), 
 and E0241 (gfp).

Top10 Transformation

 Thaw cells; aliquot out ~30uL per transformation.
 Add desired amount of plasmid DNA (1 uL in this case?) to cells and leave on ice for 30 min.
 Heat shock at 37&degC for 30 sec in a heating block.  
 Ice for 2 min.
 Shake in 37%deg incubator for 1 hr.
 Pour onto plates with the required antibiotic (carbinomycin in this case); spread with beads or plate spreader.
 Incubate plates in 37%deg incubator overnight.


HOMEWORK:

  1. Past iGEM Projects Presentation: UCSF
  2. Prospective Projects Research:
 Lactobacillus Hijacking
 Receptor-based DNA Nanostructure Latch 
 Biocryptography w/ DNA Nanostructures
 ?

Contact Info

/Tiffany Chan /Cell: 781 330 1969 /chan.tiffany at gmail.com

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