User:TChan: Difference between revisions
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M 6.12.06 | == | ||
==M 6.12.06== | |||
1. Folded DNA Nanostructures | |||
<b>Goal:</b> Create nanotubes using Shawn's pre-designed oligo staples, and two controls: one without the scaffold, one without the staples | |||
*According to [http://openwetware.org/wiki/Folding_DNA_nanostructures protocol], 3 combinations of components were mixed and thermocycled. | |||
2. Transformed Bacteria with Existing BioBrick Plasmids | |||
<b>Goal:</b> Transform existing BioBrick-containing plasmids in order to produce large enough quantities of DNA to work with later. | |||
*R0010 (lac operon promoter), | |||
*E7104 (T4 promoter + gfp), | |||
*and E0241 (gfp) | |||
were transformed into OneShot Top10 competent cells (Invitrogen) according to | |||
HOMEWORK: | HOMEWORK: | ||
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d) ? | d) ? | ||
==Email== | |||
[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com] | |||
Revision as of 11:49, 14 June 2006
==
M 6.12.06
1. Folded DNA Nanostructures Goal: Create nanotubes using Shawn's pre-designed oligo staples, and two controls: one without the scaffold, one without the staples
- According to protocol, 3 combinations of components were mixed and thermocycled.
2. Transformed Bacteria with Existing BioBrick Plasmids Goal: Transform existing BioBrick-containing plasmids in order to produce large enough quantities of DNA to work with later.
- R0010 (lac operon promoter),
- E7104 (T4 promoter + gfp),
- and E0241 (gfp)
were transformed into OneShot Top10 competent cells (Invitrogen) according to
HOMEWORK:
1) Past iGEM Projects Presentation: UCSF
2) Prospective Projects Research:
a) Lactobacillus Hijacking
b) Receptor-based DNA Nanostructure Latch
c) Biocryptography w/ DNA Nanostructures
d) ?
