User:TChan: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
== | ==W 6.14.06== | ||
1. | |||
==Tu 6.13.06== | ==Tu 6.13.06== | ||
1. Picked Colonies of Existing BioBrick Transformants | 1. Picked Colonies of Existing BioBrick Transformants | ||
| Line 7: | Line 7: | ||
2 colonies of E0, | 2 colonies of E0, | ||
2 colonies of R0, and numbered accordingly | 2 colonies of R0, and numbered accordingly | ||
*Grew each colony in 5mL LB + 5uL of 50mg/mL Amp (1:100 dilution). | |||
2. Ran Gel of DNA Nanostructures | 2. Ran Gel of DNA Nanostructures | ||
| Line 13: | Line 14: | ||
1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold | 1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold | ||
| Line 26: | Line 27: | ||
==M 6.12.06== | ==M 6.12.06== | ||
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA | 1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA | ||
*Three reactions were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures given protocol]. | *Three reactions below were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures given protocol]. | ||
oligos + scaffold | oligos + scaffold | ||
-oligos | -oligos | ||
| Line 32: | Line 33: | ||
2. Transformed Bacteria with Existing BioBrick Plasmids | 2. Transformed Bacteria with Existing BioBrick Plasmids | ||
*Transformed into OneShot Top10 competent cells (Invitrogen) according to the following protocol. | *Transformed each BioBrick plasmid below into OneShot Top10 competent cells (Invitrogen) according to the following protocol. | ||
R0010 (lac operon promoter), | R0010 (lac operon promoter), | ||
E7104 (T4 promoter + gfp), | E7104 (T4 promoter + gfp), | ||
| Line 48: | Line 49: | ||
HOMEWORK: | HOMEWORK: | ||
#Past iGEM Projects Presentation: UCSF | |||
#Prospective Projects Research: | |||
Lactobacillus Hijacking | |||
Receptor-based DNA Nanostructure Latch | |||
Biocryptography w/ DNA Nanostructures | |||
? | |||
== | ==Contact Info== | ||
[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com] | /Tiffany Chan | ||
/Cell: 781 330 1969 | |||
/[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com] | |||
Revision as of 12:50, 14 June 2006
W 6.14.06
1.
Tu 6.13.06
1. Picked Colonies of Existing BioBrick Transformants
- Picked:
3 colonies of E7, 2 colonies of E0, 2 colonies of R0, and numbered accordingly
- Grew each colony in 5mL LB + 5uL of 50mg/mL Amp (1:100 dilution).
2. Ran Gel of DNA Nanostructures
- Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V. (NB: Only left four lanes are ours.)
1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold
M 6.12.06
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA
- Three reactions below were mixed according to given protocol.
oligos + scaffold -oligos -scaffold
2. Transformed Bacteria with Existing BioBrick Plasmids
- Transformed each BioBrick plasmid below into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
R0010 (lac operon promoter), E7104 (T4 promoter + gfp), and E0241 (gfp).
Top10 Transformation
Thaw cells; aliquot out ~30uL per transformation. Add desired amount of plasmid DNA (1 uL in this case?) to cells and leave on ice for 30 min. Heat shock at 37°C for 30 sec in a heating block. Ice for 2 min. Shake in 37%deg incubator for 1 hr. Pour onto plates with the required antibiotic (carbinomycin in this case); spread with beads or plate spreader. Incubate plates in 37%deg incubator overnight.
HOMEWORK:
- Past iGEM Projects Presentation: UCSF
- Prospective Projects Research:
Lactobacillus Hijacking Receptor-based DNA Nanostructure Latch Biocryptography w/ DNA Nanostructures ?
Contact Info
/Tiffany Chan /Cell: 781 330 1969 /chan.tiffany at gmail.com
