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Wed 6.14.06

Tu 6.13.06

1. Picked Colonies of Existing BioBrick Transformants

  • Picked:
 3 colonies of E7, 
 2 colonies of E0, 
 2 colonies of R0, and numbered accordingly

2. Ran Gel of DNA Nanostructures

  • Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V. (NB: Only left four lanes are ours.)
Nanostructure Gel
Nanostructure Gel
 1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold

M 6.12.06

1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA

oligos + scaffold

2. Transformed Bacteria with Existing BioBrick Plasmids

  • Transformed into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
 R0010 (lac operon promoter),
 E7104 (T4 promoter + gfp), 
 and E0241 (gfp).

Top10 Transformation

 Thaw cells; aliquot out ~30uL per transformation.
 Add desired amount of plasmid DNA (1 uL in this case?) to cells and leave on ice for 30 min.
 Heat shock at 37&degC for 30 sec in a heating block.  
 Ice for 2 min.
 Shake in 37%deg incubator for 1 hr.
 Pour onto plates with the required antibiotic (carbinomycin in this case); spread with beads or plate spreader.
 Incubate plates in 37%deg incubator overnight.

HOMEWORK: 1) Past iGEM Projects Presentation: UCSF 2) Prospective Projects Research: a) Lactobacillus Hijacking b) Receptor-based DNA Nanostructure Latch c) Biocryptography w/ DNA Nanostructures d) ?

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