User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/10/01

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Oct 1 - Diffusion

  • Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad)
  • Group Name : AMT

Objective

Two groups will be working on determining fluorophore concentration. (Alicia/Madeleine and Becky/Eleni/Melvin) The other two groups will be working on diffusion. (Jake/James/Mary and Andrew/Michael/Tami).

NOTE: The groups working on fluorophores will need to space to the groups working on diffusion as their research is very time sensitive.

Description

Fluorescence

  1. Make stock concentrations (both groups can use the same solutions)
    1. 0.10uM
    2. 0.50uM
    3. 1.0uM
    4. 1.5uM
    5. 2.0uM
  2. Take UV-Vis and Fluorescence spectra of these samples.
  3. Make a calibration curve based on UV-Vis.
    1. Compare your data to some published values
  4. Make a calibration curve based on the fluorescence.
    1. In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.

Diffusion

  1. One group will use a PVA film
  2. The other group will use a PVA/clay film
  3. Set the film between the two chambers
  4. Add liquid to each chamber
    1. One chamber gets water
    2. The other chamber gets 20ppm Malachite green
  5. Withdraw 200uL from each chamber at the 5 minute mark and place in their own eppy tube
    1. NOTE: I want the groups to coordinate so that their 5 minutes don't overlap with one another.
    2. NOTE: You'll want to use a small volume cuvette
    3. NOTE: You'll also want a 0minute measurement for each.
    4. NOTE: You'll likely have to dilute the sample from your chamber with the 20ppm. So, when you return the sample, only return the non-diluted sample.
  6. Measure the UV-Vis spectrum for both samples. Return each to the chamber it originally came from
  7. Take measurements at the following times
    1. 15 minutes
    2. 30 minutes
    3. 1 hour
    4. 2 hours
    5. right before you leave lab
    6. It would be great if someone could stop by on Thursday to take a measurement then.
    7. You'll be taking measurements on Friday too.
  8. During each of this, it would be best to start working up the data right away.

The description is from Dr. Hartings Notebook


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