User:Tamra L. Fisher/Notebook/Research for Dr. Hartings/2012/07/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Reconstitute Asc Hb with Nickel==
# Ni(protoporphyrin IX)solution was added again dropwise (pretty slowly) to the apoprotein (while gently stirring).
# Ni(protoporphyrin IX)solution was added again dropwise (pretty slowly) to the apoprotein (while gently stirring).
#* The Ni(protoporphyrin IX)solution was prepared yesterday by dissolving 2mg of Ni(protoporphyrin IX) in 10 drops of 0.1N NaOH, and then adding 4.5mL of dH<sub>2</sub>O. 2mL of 0.1M Potassium Phosphate buffer (pH 7.1) was added to this solution. The solution was mixed on a stir plate.
#* The Ni(protoporphyrin IX)solution was prepared yesterday by dissolving 2mg of Ni(protoporphyrin IX) in 10 drops of 0.1N NaOH, and then adding 4.5mL of dH<sub>2</sub>O. 2mL of 0.1M Potassium Phosphate buffer (pH 7.1) was added to this solution. The solution was mixed on a stir plate.

Revision as of 06:31, 16 July 2012

Asc Hb Project <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Reconstitute Asc Hb with Nickel

  1. Ni(protoporphyrin IX)solution was added again dropwise (pretty slowly) to the apoprotein (while gently stirring).
    • The Ni(protoporphyrin IX)solution was prepared yesterday by dissolving 2mg of Ni(protoporphyrin IX) in 10 drops of 0.1N NaOH, and then adding 4.5mL of dH2O. 2mL of 0.1M Potassium Phosphate buffer (pH 7.1) was added to this solution. The solution was mixed on a stir plate.
  2. The beaker with the apoprotein/Ni(protoporphyrin IX) mixture was wrapped in aluminum foil and left to gently stir in the cold room for 12 hours.
  3. 12 hours later the stir plate was turned off so the apoprotein/Ni(protoporphyrin IX) mixture could sit undisturbed overnight.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tamra_L._Fisher/Notebook/Research_for_Dr._Hartings/2012/07/15&oldid=614219"