User:Tara K. Luckau/Notebook/Team ConGen/2010/09/27: Difference between revisions
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== | ==Ordered Primers== | ||
* | * Ordered first set of primers | ||
* | * Scun2 and Scun22 (highly variable simple motifs) | ||
** in | ** Lance et al (2009) Conservation Genetics Resources, ''Sceloporus undulatus'' | ||
# | * Forward and reverse as given in publication, also reverse with pigtail (GTTTCTT) at 5' end, also forward with M13F(-21) tag (TGTAAAACGACGGCCAGT) | ||
# | * [[Image:Luckau_Primer20100927.jpg|500 px]] | ||
* Try all four combinations | |||
# F - R | |||
* | # F - R pigtail | ||
# F M13 - R | |||
# F M13 - R pigtail | |||
* If they don't hinder efforts or change optimization decisions, then may make optimization of multiplexes cheaper! | |||
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Revision as of 17:11, 28 October 2010
 Tara's Lab Notebook
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Ordered Primers
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