User:Tara K. Luckau/Notebook/Team ConGen/2010/10/29: Difference between revisions
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== | ==Thoughts and Musings== | ||
* | ===DNA Concentration=== | ||
* | * Tina is prepping mass DNA extraction for optimization (using extra samples with lots of chunky) | ||
** | * Want to use these samples to figure out relative DNA concentration to use for all future PCR optimizations | ||
* Take Tina's extractions and spot checks | |||
* Run PCR on DNA serial dilution; gel for which concentrations produced product | |||
* note smallest concentration that worked | |||
=== | * shove it through NanoDrop | ||
* | * Compare that sample's spot check and NanoDrop - that's the brightness of spot we'll want for all future PCRs | ||
===Questions=== | |||
* since I don't really have a verified working primer set, how can I expect the PCR to work? | |||
* will necessarily need to run DNA concentration gradient, temp gradient and buffer gradient in same reaction | |||
Revision as of 13:18, 30 October 2010
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Thoughts and MusingsDNA Concentration
Questions
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