User:Tara K. Luckau/Notebook/Team ConGen/2010/11/08: Difference between revisions
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* 27µL Gel Red (added when agarose turned clear but still hot, used stir bar) | * 27µL Gel Red (added when agarose turned clear but still hot, used stir bar) | ||
===Load Gel=== | ===Load Gel=== | ||
* 5µL loading dye | * 5µL NanoPure H<sub>2</sub>O + 5µL sample (to conserve loading dye) (don't need loading dye in every sample since it's in ladder) | ||
* 10µL ladder / loading dye mix | |||
* [[Image:20101108 GelSchematic.jpg|800 px]] | * [[Image:20101108 GelSchematic.jpg|800 px]] | ||
===Run Gel=== | ===Run Gel=== | ||
* 130C, 2 hrs | * 130C, 2 hrs | ||
Revision as of 15:38, 8 November 2010
 Tara's Lab Notebook
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Gel Scun2 F-R with DNA GradientCast Gel
Load Gel
Run Gel
Image GelResults
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