User:Tara K. Luckau/Notebook/Team ConGen/2010/11/09: Difference between revisions
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== | ==Meeting with Rulon - PCR fixes== | ||
* | # try Scun22 primers | ||
* | #* maybe Scun2 just doesn't cross-amplify between ''Sceloporus undulatus'' and ''Sceloporus occidentalis'' | ||
** | # check math on dNTPs, ladder (too bright?) | ||
# | #* maybe I didn't make them correctly | ||
# | # check pH of Tris-Cl and Low TE | ||
#* the current working stocks of both are being stored in 50mL conicals instead of glass | |||
# try positive PCR product straight into gel | |||
* | #* is my ladder too concentrated or is my DNA not amplifying | ||
# PCR Rulon's timber rattlers | |||
#* Box "C. horridus Primer stocks" | |||
#** use #9 (needs rehydration) | |||
#** use his Plat Taq, 15mM MgCl<sub>2</sub>, 10x PCR Buffer, dNTPmix | |||
#* Box "C. horridus NH DNA extract 9-17-8" | |||
#** extracted DNA, but from different locale | |||
#** NanoDrop before using (don't know concentrations) | |||
# try better quality Taq | |||
#* may need higher fidelity, or ice wasn't enough | |||
Revision as of 17:37, 9 November 2010
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Meeting with Rulon - PCR fixes
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