User:Tara K. Luckau/Notebook/Team ConGen/2011/01/11: Difference between revisions
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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | |style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | ||
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* Try to break the PCR by using Tara's dNTPs, Tara's Buffer | * Try to break the PCR by using Tara's dNTPs, Tara's Buffer | ||
* Try to make Scun PCRs work by using Rulon's dNTPs, 10x Buffer + MgCl<sub>2</sub> | * Try to make Scun PCRs work by using Rulon's dNTPs, 10x Buffer + MgCl<sub>2</sub> | ||
==Extract Sceloporus undulatus== | |||
* acquired from Tod Reeder; "H0074; TWR 78" (looks like muscle and liver tissue) | |||
* extract same species as primers were designed for, to rule out DNA extraction and primer synthesis as PCR problem | |||
# Add 300µL of Cell Lysis Solution to about 10mg tissue | |||
# Add 2µL Proteinase K (20mg/mL); incubate overnight at 55°C water bath | |||
Revision as of 12:29, 12 January 2011
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Gel Touchdown
Gel Timber Rattlesnakes
Next Steps
Extract Sceloporus undulatus
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