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 Tara's Lab Notebook
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Gel Touchdown
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 - Faint bands at very bottom, near next rows' lanes = primer front
- ladder triplet: 396/344/298
- ladder doublet: 220/201
- ladder doublet: 154/134
- ladder band: 75
- but no amplicon at expected 450bp
Gel Timber Rattlesnakes
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- Definitely bands! Too bad I ran off primer 10's bands (but I assume they're there)
Next Steps
- Try to break the PCR by using Tara's dNTPs, Tara's Buffer
- Try to make Scun PCRs work by using Rulon's dNTPs, 10x Buffer + MgCl2
- acquired from Tod Reeder; "H0074; TWR 78" (looks like muscle and liver tissue)
- extract same species as primers were designed for, to rule out DNA extraction and primer synthesis as PCR problem
- Add 300µL of Cell Lysis Solution to about 10mg tissue
- Add 2µL Proteinase K (20mg/mL); incubate overnight at 55°C water bath
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Tara's Lab Notebook
