User:Tara K. Luckau/Notebook/Team ConGen/2011/01/14: Difference between revisions

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==Differences between Lance conditions and previous trials==
{| border="1"
| || 10January2011 (fail) || 13January2011 (worked)
|-
| Buffer / MgCl<sub>2</sub> || 1.5 || 1
|-
| Primers || 0.1 each || 0.25 each
|-style="background: yellow"
| Taq || 0.05 (0.25 Units/reaction)|| 0.5 (2.5 Units/reaction)
|}
The largest difference between the two reactions was Taq - 10-fold difference
In the interest of cost, try to pull this down to 1 Unit/reaction (0.2µL)




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|}


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Revision as of 14:27, 14 January 2011

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Gel Lance conditions

Success!!!



  • Scun2 Primer - expecting bands ~150bp
    • Scun sample - band at 130bp
    • Scoc sample - band at 75bp


  • Scun22 Primer - expecting bands at ~450bp
    • Scun sample - multiple bands; but there is one prominent band in one of the replicates at 450bp
    • Scoc sample - multiple bands; but prominent band in all three replicates at 450bp


Differences between Lance conditions and previous trials

10January2011 (fail) 13January2011 (worked)
Buffer / MgCl2 1.5 1
Primers 0.1 each 0.25 each
Taq 0.05 (0.25 Units/reaction) 0.5 (2.5 Units/reaction)

The largest difference between the two reactions was Taq - 10-fold difference In the interest of cost, try to pull this down to 1 Unit/reaction (0.2µL)



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