User:Tara K. Luckau/Notebook/Team ConGen/2011/01/14: Difference between revisions
From OpenWetWare
(One intermediate revision by the same user not shown) | |||
Line 44: | Line 44: | ||
The largest difference between the two reactions was Taq - 10-fold difference | The largest difference between the two reactions was Taq - 10-fold difference | ||
In the interest of cost, try to pull this down to 1 Unit/reaction (0.2µL) | * In the interest of cost, try to pull this down to 1 Unit/reaction (0.2µL) | ||
* Also re-calculate for 10µL reaction volume | |||
* Find annealing temperatures (touchdowns are poopy) | |||
==PCR decrease Taq== | |||
* [[Image:20110114_PCROpt_Touchdown.jpg|900 px]] | |||
Revision as of 16:24, 14 January 2011
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||
Gel Lance conditionsSuccess!!!
Differences between Lance conditions and previous trials
The largest difference between the two reactions was Taq - 10-fold difference
PCR decrease Taq
|