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 Tara's Lab Notebook
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Gel Lance conditions
Success!!!
 - Scun2 Primer - expecting bands ~150bp
- Scun sample - band at 130bp
- Scoc sample - band at 75bp
 - Scun22 Primer - expecting bands at ~450bp
- Scun sample - multiple bands; but there is one prominent band in one of the replicates at 450bp
- Scoc sample - multiple bands; but prominent band in all three replicates at 450bp
Differences between Lance conditions and previous trials
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10January2011 (fail) |
13January2011 (worked)
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| Buffer / MgCl2 |
1.5 µL |
1 µL
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| Primers |
0.1 µL each |
0.25 µL each
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| Taq |
0.05 µL (0.25 Units) |
0.5 µL (2.5 Units)
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The largest difference between the two reactions was Taq - 10-fold difference
- In the interest of cost, try to pull this down to 1 Unit/reaction (0.2µL)
- Also re-calculate for 10µL reaction volume
- Find annealing temperatures (touchdowns are poopy)
PCR decrease Taq
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Tara's Lab Notebook
