User:Tara K. Luckau/Notebook/Team ConGen/2011/02/21

From OpenWetWare
Jump to navigationJump to search
Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Scun22 PCRs from 7 and 10 February

  • These are from the PCRs that Rulon and I demo'd to the students
  • In both cases, used Scun22 primers, run on both Scoc and Scun DNA.
  • We used the same PCR sheet, but Rulon put his samples in A12-H12 of his PCR plate. Same reaction volumes used.
  • Rulon PCR'd 7 Feb 2011; Tara PCR'd 10 Feb 2011


Scun22 Agarose Gel

  • Pour small Gel: 50 mL 1x TAE + 1 g agarose + 5 µL GelRed
  • Load: 5µL Orange/Blue loading dye + 2 µL PCR product
  • This is the first time I'm trying the Orange/Blue Loading Dye (Orange G and Xylene Cyanol FF), stolen from Joe Newsome's excess stock
  • Run: 80V, 40 min




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2011/02/21&oldid=494704"