User:Tara K. Luckau/Notebook/Team ConGen/2011/03/04: Difference between revisions

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==Scun22 Optimization PCR Gel==
==Scun22 Optimization PCR Gel==
* from 23 february 2011 PCR (full temperature and buffer gradient)
* from 23 february 2011 PCR (full temperature and buffer gradient)
===Gel Specs===
* Pour: 270mL 1xTAE  +  5g agarose (supposed to have been 5.4g)  +  27µL GelRed
* Pour: 270mL 1xTAE  +  5g agarose (supposed to have been 5.4g)  +  27µL GelRed
* Run: 40 min @ 110V
* Run: 40 min @ 110V
*


===Gel Images===
* [[Image:20110304_Row1.TIF|750 px]]
* [[Image:20110304_Row2.TIF|750 px]]
* [[Image:20110304_Row3.TIF|750 px]]
* Only Buffer G yielded any product ... zoom in:
* [[Image:20110304a_Row3Right.jpg|750 px]]
===Conclusions===
* doesn't look like a typical MgCl<sub>2</sub> and temperature gradient PCR
* inconsistent banding patterns across temperatures with dropouts
* regardless, there is mispriming in all conditions that yielded any product
* <span style="background:#7CFC00">Scun22 might not be a primer that will work for Sceloporus occidentalis</span>


===Subsection===
* here






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Revision as of 17:22, 4 March 2011

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Scun22 Optimization PCR Gel

  • from 23 february 2011 PCR (full temperature and buffer gradient)

Gel Specs

  • Pour: 270mL 1xTAE + 5g agarose (supposed to have been 5.4g) + 27µL GelRed
  • Run: 40 min @ 110V

Gel Images


  • Only Buffer G yielded any product ... zoom in:

Conclusions

  • doesn't look like a typical MgCl2 and temperature gradient PCR
  • inconsistent banding patterns across temperatures with dropouts


  • regardless, there is mispriming in all conditions that yielded any product
  • Scun22 might not be a primer that will work for Sceloporus occidentalis
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