User:Tara K. Luckau/Notebook/Team ConGen/2011/06/07

From OpenWetWare

< User:Tara K. Luckau | Notebook | Team ConGen | 2011 | 06
Revision as of 19:52, 7 June 2011 by Tara K. Luckau (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


Scun10

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 1 µL gel load dye + 5 µL PCR product 160 V
27 µL GelRed 6 µL ladder 1 hour


  • double banding noticeable under majority of PCR conditions; I'm assuming this is attributable to diploid nuclear DNA - in this case, a large enough difference in repeats between the two copies that it's clearly visible on an agarose gel


Scun11

PCR

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 1 µL gel load dye + 5 µL PCR product 140 V
27 µL GelRed 6 µL ladder 1.5 hour


Scun14

PCR



Personal tools