User:Tara K. Luckau/Notebook/Team ConGen/2011/06/22: Difference between revisions
From OpenWetWare
(→Gel) |
No edit summary |
||
(One intermediate revision by the same user not shown) | |||
Line 9: | Line 9: | ||
==Multiplex 1== | ==SCOC Multiplex 1== | ||
* SCOC multiplex diversity panel with labelled primers to send to frag! Woot! | * SCOC multiplex diversity panel with labelled primers to send to frag! Woot! | ||
Line 39: | Line 39: | ||
===Next Step=== | ===Next Step=== | ||
* re-do PCR, but | * re-do PCR, but have 8-well strip with 0.8*4 µL DNA plus Big MM, then transfer from Mini MM straight across into PCR plate | ||
* might also be worth doing a PCR similar to that from 13 June 2011 - uniplex of each primer pair, plus multiplex - to 'test' labeled primers | * might also be worth doing a PCR similar to that from 13 June 2011 - uniplex of each primer pair, plus multiplex - to 'test' labeled primers | ||
* over night, test full-skirt PCR plate with flat-top 8-well strips to see how much evaporation occurs - this is how I plan to ship for frag | * over night, test full-skirt PCR plate with flat-top 8-well strips to see how much evaporation occurs - this is how I plan to ship for frag |
Revision as of 14:53, 23 June 2011
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||
SCOC Multiplex 1
PCR
Gel
Next Step
|