User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23: Difference between revisions
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* similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers | * similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers | ||
* [[Image:20110623 PCR.jpg|800 px]] | * [[Image:20110623 PCR.jpg|800 px]] | ||
===Gel=== | |||
* used left-over gel from 13 June 2011 | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Load !! Run | |||
|- | |||
| 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 6 µL ladder || 1 hour | |||
|} | |||
* see 13 June 2011 for banding pattern expectations | |||
* [[Image:20110623 Gel.jpg|600 px]] | |||
* a whole lotta nada :( | |||
Revision as of 09:14, 23 June 2011
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MP1PCR
Gel
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