User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23: Difference between revisions

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* similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers
* similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers
* [[Image:20110623 PCR.jpg|800 px]]
* [[Image:20110623 PCR.jpg|800 px]]
===Gel===
* used left-over gel from 13 June 2011
{| {{table}} style="text-align: center"
|-
! Load !! Run
|-
| 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 6 µL ladder || 1 hour
|}
* see 13 June 2011 for banding pattern expectations
* [[Image:20110623 Gel.jpg|600 px]]
* a whole lotta nada  :(




Revision as of 09:14, 23 June 2011

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MP1

PCR

  • similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers


Gel

  • used left-over gel from 13 June 2011
Load Run
2 µL gel load dye + 4 µL PCR product 160 V
6 µL ladder 1 hour


  • see 13 June 2011 for banding pattern expectations
  • a whole lotta nada  :(



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