User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23: Difference between revisions
From OpenWetWare
(→Gel) |
No edit summary |
||
(8 intermediate revisions by the same user not shown) | |||
Line 9: | Line 9: | ||
== | ==SCOC Multiplex 1== | ||
Line 15: | Line 15: | ||
* similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers | * similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers | ||
* [[Image:20110623 PCR.jpg|800 px]] | * [[Image:20110623 PCR.jpg|800 px]] | ||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Pour !! Load !! Run | |||
|- | |||
| 270 mL 1x TAE + 5.4 g agarose || 1 µL gel load dye + 5 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 50 minutes | |||
|} | |||
* see 13 June 2011 for banding pattern expectations | |||
* [[Image:20110623 Gel.jpg|800 px]] | |||
* consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there) | |||
* notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient | |||
==SCOC Multiplex 1== | |||
* SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux! | |||
===PCR=== | |||
* [[Image:20110623 PCR2.jpg|800 px]] | |||
* some of the samples used for the diversity panel are getting low ... should switch out soon | |||
* SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06 | |||
Line 30: | Line 58: | ||
* see 13 June 2011 for banding pattern expectations | * see 13 June 2011 for banding pattern expectations | ||
* [[Image:20110623 | * [[Image:20110623 Gel2.jpg|600 px]] | ||
* a whole lotta nada :( | * still a whole lotta nada :( | ||
* strong primer band, again | |||
* a bit of amplification of Scun9/10/11, but it's crap | |||
===Next Steps=== | |||
* try full gradient on multiplex, ugh | |||
Revision as of 09:28, 24 June 2011
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||
SCOC Multiplex 1PCR
Gel
SCOC Multiplex 1
PCR
Gel
Next Steps
|