User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23: Difference between revisions
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== | ==SCOC Multiplex 1== | ||
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! Pour !! Load !! Run | ! Pour !! Load !! Run | ||
|- | |- | ||
| 270 mL 1x TAE + 5.4 g agarose || | | 270 mL 1x TAE + 5.4 g agarose || 1 µL gel load dye + 5 µL PCR product || 160 V | ||
|- | |- | ||
| 27 µL GelRed || 6 µL ladder || | | 27 µL GelRed || 6 µL ladder || 50 minutes | ||
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* notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient | * notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient | ||
==SCOC Multiplex 1== | |||
* SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux! | |||
===PCR=== | |||
* [[Image:20110623 PCR2.jpg|800 px]] | |||
* some of the samples used for the diversity panel are getting low ... should switch out soon | |||
* SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06 | |||
===Gel=== | |||
* used left-over gel from 13 June 2011 | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Load !! Run | |||
|- | |||
| 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 6 µL ladder || 1 hour | |||
|} | |||
* see 13 June 2011 for banding pattern expectations | |||
* [[Image:20110623 Gel2.jpg|600 px]] | |||
* still a whole lotta nada :( | |||
* strong primer band, again | |||
* a bit of amplification of Scun9/10/11, but it's crap | |||
===Next Steps=== | |||
* try full gradient on multiplex, ugh | |||
Revision as of 09:28, 24 June 2011
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SCOC Multiplex 1PCR
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SCOC Multiplex 1
PCR
Gel
Next Steps
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