User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23

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SCOC Multiplex 1

PCR

  • similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 1 hour


  • see 13 June 2011 for banding pattern expectations
  • consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there)
  • notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient


SCOC Multiplex 1

  • SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux!

PCR


Gel

  • used left-over gel from 13 June 2011
Load Run
2 µL gel load dye + 4 µL PCR product 160 V
6 µL ladder 1 hour



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