SCOC Multiplex 1
PCR
- similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers
Gel
Pour |
Load |
Run
|
270 mL 1x TAE + 5.4 g agarose |
1 µL gel load dye + 5 µL PCR product |
160 V
|
27 µL GelRed |
6 µL ladder |
50 minutes
|
- see 13 June 2011 for banding pattern expectations
- consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there)
- notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient
SCOC Multiplex 1
- SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux!
PCR
- some of the samples used for the diversity panel are getting low ... should switch out soon
- SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06
Gel
- used left-over gel from 13 June 2011
Load |
Run
|
2 µL gel load dye + 4 µL PCR product |
160 V
|
6 µL ladder |
1 hour
|
- see 13 June 2011 for banding pattern expectations
- still a whole lotta nada :(
- strong primer band, again
- a bit of amplification of Scun9/10/11, but it's crap
Next Steps
- try full gradient on multiplex, ugh
|