User:Tara K. Luckau/Notebook/Team ConGen/2011/07/07: Difference between revisions
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: gel band size tends to be on the small end of expected amplicon size based on Lance's paper | : gel band size tends to be on the small end of expected amplicon size based on Lance's paper | ||
: frag peaks don't align with gel band size - difference in migration between agarose gel and capillary? | : frag peaks don't align with gel band size - difference in migration between agarose gel and capillary? | ||
: blue lane amplicons groups (Scun2 and Scun10) seem like they are likely, given the species difference (primers developed on Sceloporus undulatus; amplified here on Sceloporus occidentalis) | : blue lane amplicons groups (Scun2 and Scun10) seem like they are likely, given the species difference | ||
::(primers developed on Sceloporus undulatus; amplified here on Sceloporus occidentalis) | |||
: green lane amplicons group is far outside the expected size range of Scun11 and far different from the agarose gel band size | : green lane amplicons group is far outside the expected size range of Scun11 and far different from the agarose gel band size | ||
:: however, consistent with Scun15 (labeled with HEX) expectations and gel band | :: however, consistent with Scun15 (labeled with HEX) expectations and gel band |
Revision as of 13:01, 8 July 2011
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Data Confusion
The Evidence
The Possible Explanation
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