User:Tara K. Luckau/Notebook/Team ConGen/2011/09/26: Difference between revisions

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[[Image:20110926_Gel.png|800 px]]
[[Image:20110926_Gel.png|800 px]]
==Primer Rehydrations==
* Received next batch of primers from IDT 21Sept2011
* Rehydrated using LowTE (both F and R):
:: Scun7
:: Scun16
:: Scun19
:: sar84
:: SA52.33
:: SA60.02
* in prep for a flurry of full gradient PCRs!




Revision as of 15:17, 26 September 2011

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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sar80

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes



Primer Rehydrations

  • Received next batch of primers from IDT 21Sept2011
  • Rehydrated using LowTE (both F and R):
Scun7
Scun16
Scun19
sar84
SA52.33
SA60.02
  • in prep for a flurry of full gradient PCRs!



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