User:Tara K. Luckau/Notebook/Team ConGen/2011/09/26: Difference between revisions

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(Autocreate 2011/09/26 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==Task==
==sar80==
* here
* here
** in here
# numbered
# again




===Subsection===
===Gel===
* here
 
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20110926_Gel.png|800 px]]
 
 
==Primer Rehydrations==
* Received next batch of primers from IDT 21Sept2011
* Rehydrated using LowTE (both F and R):
:* From Lance et al. 2009 (S. undulatus)
::: Scun7
::: Scun16
::: Scun19
:* From Chan et al. 2007 (S. arenicolus)
::: sar84
::: SA52.33
::: SA60.02
* in prep for a flurry of full gradient PCRs!




Revision as of 15:21, 26 September 2011

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sar80

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes



Primer Rehydrations

  • Received next batch of primers from IDT 21Sept2011
  • Rehydrated using LowTE (both F and R):
  • From Lance et al. 2009 (S. undulatus)
Scun7
Scun16
Scun19
  • From Chan et al. 2007 (S. arenicolus)
sar84
SA52.33
SA60.02
  • in prep for a flurry of full gradient PCRs!



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