User:Tara K. Luckau/Notebook/Team ConGen/2011/09/26: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(2 intermediate revisions by the same user not shown)
Line 31: Line 31:
* Received next batch of primers from IDT 21Sept2011
* Received next batch of primers from IDT 21Sept2011
* Rehydrated using LowTE (both F and R):
* Rehydrated using LowTE (both F and R):
:: Scun7
:* From Lance et al. 2009 (S. undulatus)
:: Scun16
::: Scun7
:: Scun19
::: Scun16
:: sar84
::: Scun19
:: SA52.33
:* From Chan et al. 2007 (S. arenicolus)
:: SA60.02
::: sar84
::: SA52.33
::: SA60.02
* in prep for a flurry of full gradient PCRs!
* in prep for a flurry of full gradient PCRs!



Revision as of 15:21, 26 September 2011

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


sar80

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes



Primer Rehydrations

  • Received next batch of primers from IDT 21Sept2011
  • Rehydrated using LowTE (both F and R):
  • From Lance et al. 2009 (S. undulatus)
Scun7
Scun16
Scun19
  • From Chan et al. 2007 (S. arenicolus)
sar84
SA52.33
SA60.02
  • in prep for a flurry of full gradient PCRs!