User:Tara K. Luckau/Notebook/Team ConGen/2011/10/14: Difference between revisions

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====Best conditions====
====Best conditions====
* Scun7_25_SYRa
* Scun7_25_SYRa
:: <span style="color:#FFFFFF; background:#4169E1">Scun7 - 0.25 µL</span>
:: <span style="color:#FFFFFF; background:#4169E1">Scun7 - 0.25 µL</span>
Line 38: Line 40:
:: Scun22 - 0.2 µL
:: Scun22 - 0.2 µL
: [[Image:20111014 Frag3.png|700 px]]
: [[Image:20111014 Frag3.png|700 px]]
* Scun16_15_SYRa
* Scun16_15_SYRa
:: Scun7 - 0.2 µL
:: Scun7 - 0.2 µL
Line 44: Line 48:
:: Scun22 - 0.2 µL
:: Scun22 - 0.2 µL
: [[Image:20111014 Frag4.png|700 px]]
: [[Image:20111014 Frag4.png|700 px]]
* Scun19_30_SYRb
 
 
* Scun19_30_SYRb (but Scun19 usually did not amplify to detectable levels)
:: Scun9 - 0.3µL
:: Scun9 - 0.3µL
:: Scun15 - 0.25 µL
:: Scun15 - 0.25 µL

Revision as of 20:32, 15 October 2011

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SCOC MP1

Fragment Analysis

Summary of fragment analysis results

  • Numbers indicate intensity of peak for each marker (or average of peaks, if heterozygous)
  • At bottom, indicates ratio of each lane
Blue - Scun7:Scun10:Scun16:Scun22 intensity
Green - Scun9:Scun15:Scun19 intensity


  • Here, average of intensity for column (rounded to nearest hundred), and ratio


Best conditions

  • Scun7_25_SYRa
Scun7 - 0.25 µL
Scun10 - 0.15 µL
Scun16 - 0.2 µL
Scun22 - 0.2 µL


  • Scun16_15_SYRa
Scun7 - 0.2 µL
Scun10 - 0.15 µL
Scun16 - 0.15 µL
Scun22 - 0.2 µL


  • Scun19_30_SYRb (but Scun19 usually did not amplify to detectable levels)
Scun9 - 0.3µL
Scun15 - 0.25 µL
Scun19 - 0.3 µL