User:Tara K. Luckau/Notebook/Team ConGen/2011/10/20: Difference between revisions

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* if needed, may be able to use conditions noted by "[[Image:20111020Tri.png|20 px]]"
* if needed, may be able to use conditions noted by "[[Image:20111020Tri.png|20 px]]"
* [[Image:20111020Tri.png|20 px]] = "ehh, not great, but not horrible, either"
* [[Image:20111020Tri.png|20 px]] = "ehh, not great, but not horrible, either"


==CNHY Acos3==
==CNHY Acos3==
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* favorable conditions noted with [[Image:greenhappyface.png]]
* favorable conditions noted with [[Image:greenhappyface.png]]


==CNHY Acos5==
==CNHY Acos5==
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===PCR===
===PCR===
* [[Image:20111020_PCRb.png|800 px]]
* [[Image:20111020_PCRb.png|800 px]]
===Gel===
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
[[Image:20111020_Gelc.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY




Revision as of 21:50, 20 October 2011

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CNHY Acos2

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not preferable for CNHY
  • if needed, may be able to use conditions noted by ""
  • = "ehh, not great, but not horrible, either"


CNHY Acos3

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • favorable conditions noted with


CNHY Acos5

PCR


CNHY Acos6

PCR



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