User:Tara K. Luckau/Notebook/Team ConGen/2011/10/24: Difference between revisions

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(Autocreate 2011/10/24 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==Task==
==CNHY Acos6==
* here
 
* here
 
** in here
===Gel===
# numbered
 
# again
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V ... 80 V
|-
| 27 µL GelRed || 6 µL ladder || 25 min ... 1.5 hr
|}
 
 
[[Image:20111024_Gel.png|800 px]]
 
* lots of mispriming (fuzzy bands because had to let run for nearly 2 hours), but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
==CNHY Ai5013==
 
 
===PCR===
* [[Image:20111024_PCR.png|800 px]]
 




===Subsection===
* here




Revision as of 13:06, 27 October 2011

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CNHY Acos6

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V ... 80 V
27 µL GelRed 6 µL ladder 25 min ... 1.5 hr


  • lots of mispriming (fuzzy bands because had to let run for nearly 2 hours), but may be able to use conditions indicated by orange face (), if needed, under stringent conditions

CNHY Ai5013

PCR





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