User:Tara K. Luckau/Notebook/Team ConGen/2011/10/24: Difference between revisions
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==CNHY | ==CNHY Acos6== | ||
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! Pour !! Load !! Run | ! Pour !! Load !! Run | ||
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | | 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V ... 80 V | ||
|- | |- | ||
| 27 µL GelRed || 6 µL ladder || 25 min | | 27 µL GelRed || 6 µL ladder || 25 min ... 1.5 hr | ||
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[[Image:20111024_Gel.png|800 px]] | [[Image:20111024_Gel.png|800 px]] | ||
* lots of mispriming (fuzzy bands because had to let run for nearly 2 hours), but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png]]), if needed, under stringent conditions | * lots of mispriming (fuzzy bands because had to let run for nearly 2 hours), but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | ||
==CNHY Ai5013== | ==CNHY Ai5013== | ||
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===PCR=== | ===PCR=== | ||
* [[Image: | * [[Image:20111024_PCR.png|800 px]] | ||
Revision as of 13:06, 27 October 2011
 Tara's Lab Notebook
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CNHY Acos6Gel
CNHY Ai5013PCR
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