User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06

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(Autocreate 2012/01/06 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNHY Acos5==
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==CNHY Multiplex==
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===PCR===
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* prepping to start multiplexing CNHY primers ... so see primer interactions ...
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* [[Image:20111023_PCRa.png|800 px]]
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===Gel===
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===AutoDimer===
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* [[Image:20120106_AutoDimer.png|800 px]]
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{| {{table}} style="text-align: center"
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|-
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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[[Image:20111023_Gel.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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Revision as of 17:46, 6 January 2012

Tara's Lab Notebook Main project page
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Contents


CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


AutoDimer



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