User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 13: Line 13:
* prepping to start multiplexing CNHY primers ... so see primer interactions ...
* prepping to start multiplexing CNHY primers ... so see primer interactions ...
 +
 +
 +
===Gradient Summary===
 +
 +
 +
* green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
 +
* [[Image:20120106 GradientSummary.png]]
 +
 +
 +
===Multiplex Map===
 +
 +
 +
* markers placed arbitrarily
 +
* marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
 +
* [[Image:20120106 Map.png]]

Revision as of 16:58, 6 January 2012

Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
  • Image:20120106 GradientSummary.png


Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
  • Image:20120106 Map.png


AutoDimer

  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions



Personal tools