User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06

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===PCR - determine approximate ranges of each marker===
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===PCR - determine approximate range of each marker===
* [[Image:20120106_PCR.png|800 px]]
* [[Image:20120106_PCR.png|800 px]]

Revision as of 18:09, 6 January 2012

Tara's Lab Notebook Main project page
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Contents


CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
  • Image:20120106 GradientSummary.png


Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
  • Image:20120106 Map.png


AutoDimer

  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions


PCR - determine approximate range of each marker




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