User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06: Difference between revisions

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==CNHY Multiplex==
==ASHY Multiplex==




* prepping to start multiplexing CNHY primers ... so see primer interactions ...
* prepping to start multiplexing CNHY primers ... so see primer interactions ...
===Gradient Summary===
* green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
* [[Image:20120106 GradientSummary.png]]
===Multiplex Map===
* markers placed arbitrarily
* marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
* [[Image:20120106 Map.png]]




Line 20: Line 35:
* color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
* color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
* [[Image:20120106_AutoDimer.png|800 px]]
* [[Image:20120106_AutoDimer.png|800 px]]
===PCR - determine approximate range of each marker===
* pool high-quant DNA samples from throughout SD county:
:-- CNHY CPN 02
:-- CNHY LOM 08
:-- CNHY RAJ 01
:-- CNHY RAJ 35
:-- CNHY RAJ 42
:-- CNHY RAJ 50
:-- CNHY TP1 11
:-- CNHY TP3 05
:-- CNHY TP3 19
:-- CNHY TP3 20
* 2µL each DNA STK + 80µL LowTE (to dilute to ~10ng/µL) + 20µL CNHY 062911TKLH ('play' DNA, already 10 ng/µL)
* [[Image:20120106_PCR.png|800 px]]




Revision as of 09:40, 20 February 2013

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ASHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary


Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.


AutoDimer

  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions


PCR - determine approximate range of each marker

  • pool high-quant DNA samples from throughout SD county:
-- CNHY CPN 02
-- CNHY LOM 08
-- CNHY RAJ 01
-- CNHY RAJ 35
-- CNHY RAJ 42
-- CNHY RAJ 50
-- CNHY TP1 11
-- CNHY TP3 05
-- CNHY TP3 19
-- CNHY TP3 20
  • 2µL each DNA STK + 80µL LowTE (to dilute to ~10ng/µL) + 20µL CNHY 062911TKLH ('play' DNA, already 10 ng/µL)





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